Identification of interspecific translocation chromosomes in human-Chinese hamster hybrid cells
β Scribed by Wim Burgerhout
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 184 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
Chromosome material of human and Chinese hamster origin can be stained differentially in human-Chinese hamster translocation chromosomes by use of the Giemsa-11 staining technique.
π SIMILAR VOLUMES
About 75 man-Chinese hamster hybrid clones were analysed for their human chromosome complement and simultaneously tested for human enzyme markers. Correlation of the presence of chromosomes and enzyme activity revealed assignments of the PGD linkage group to chromosome 1, ME1, PGM a and IPO-B to 6,
## Abstract A chromosome that controls malignancy in Chinese hamster cells has been identified by analysis of the Giemsa banding pattern of a malignant cell line transformed by simian virus 40 (SV40), nonβmalignant revertants from this line, segregants from the revertants that were again malignant
## Abstract Hybrid clones were obtained between a mouse cell line (3TP) and a temperatureβsensitive Chinese hamster cell line (K12) unable to grow at 40Β° because of a __ts__ defect apparently located at the G1/S transition. The great majority of hybrid clones grew at 40Β°, showing the __ts__ defect