A simple, fast and reliable immunonephelometric (I-NEPH) method has been worked out for the determination of CSF proteins. A good quality and low price I-NEPH apparatus was used. The results were obtained from regression lines, constructed from various parts of the calibration curve, calculated by u
Simple, rapid, turbidometric determination of inorganic sulfate and/or protein
โ Scribed by S.G. Jackson; E.L. McCandless
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 342 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A simple, reproducible, turbidometric assay, adaptable to protein and inorganic sulfate analysis is described. A linear relationship between optical density and concentration is found for sulfate between 0 and 80 pg and protein between 25 and 2500 pg. Maximal turbidity develops over a relatively short time and is stable for some time (over an hour for sulfate analysis). Many compounds which interfere with other assays have no effect on this system.
๐ SIMILAR VOLUMES
In the study of protein dynamics by (13)C or (15)N relaxation measurements different models from the Lipari-Szabo formalism are used in order to determine the motion parameters. The global rotational correlation time tau(R) of the molecule must be estimated prior to the analysis. In this Communicati