Simple method for the determination of homovanillic acid and vanillylmandelic acid in urine by high-performance liquid chromatography
β Scribed by Akira Yoshida; Masanori Yoshioka; Toshio Sakai; Zenzo Tamura
- Book ID
- 108447781
- Publisher
- Elsevier Science
- Year
- 1982
- Weight
- 383 KB
- Volume
- 227
- Category
- Article
- ISSN
- 0378-4347
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π SIMILAR VOLUMES
A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau
The biomedically and neurochemically important compounds 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) have been simultaneously determined in human urine after reverse-phase two-dimensional high-performance liquid chromatography. A 10-fold-diluted urine sample (20 microliters) is f