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Analysis of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid in human urine by high-performance liquid chromatography and fluorometry

✍ Scribed by J.P.M. Wielders; Chr.J.K. Mink


Publisher
Elsevier Science
Year
1984
Weight
508 KB
Volume
310
Category
Article
ISSN
0378-4347

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πŸ“œ SIMILAR VOLUMES


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✍ G. Alemany; A. GamundΓ­; C. RossellΓ³; R. Rial πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 198 KB πŸ‘ 2 views

A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau

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The biomedically and neurochemically important compounds 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) have been simultaneously determined in human urine after reverse-phase two-dimensional high-performance liquid chromatography. A 10-fold-diluted urine sample (20 microliters) is f