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Simultaneous liquid chromatographic determination of vanillylmandelic acid, homovanillic acid, and 5-hydroxy-3-indoleacetic acid in urine, using isocratic elution and electrochemical detection

✍ Scribed by D. A. Richards; A. C. Titheradge


Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
460 KB
Volume
2
Category
Article
ISSN
0269-3879

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πŸ“œ SIMILAR VOLUMES


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✍ G. Alemany; A. GamundΓ­; C. RossellΓ³; R. Rial πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 198 KB πŸ‘ 2 views

A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau

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The biomedically and neurochemically important compounds 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) have been simultaneously determined in human urine after reverse-phase two-dimensional high-performance liquid chromatography. A 10-fold-diluted urine sample (20 microliters) is f