Simple and sensitive laser-induced fluorescence detection for capillary electrophoresis and its application to protein separation
β Scribed by Heung Bin Lim; Jong Jin Lee; Professor Kong-Joo Lee
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 422 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
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## Abstract Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laserβinduced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective a
Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laserinduced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 Ο« 10 Οͺ 12 M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.