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Detection of prion protein using a capillary electrophoresis-based competitive immunoassay with laser-induced fluorescence detection and cyclodextrin-aided separation

✍ Scribed by Wen-chu Yang; Edward S. Yeung; Mary Jo Schmerr


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
326 KB
Volume
26
Category
Article
ISSN
0173-0835

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## Abstract Carbonyl‐modified proteins are markers of oxidative damage. Here, we report a new method for detecting and quantifying carbonylated proteins by capillary sieving electrophoresis (CSE) with LIF detection (CSE‐LIF). Alexa 488 hydrazide is used for the specific labeling of carbonyls while