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Simple and convenient chemiluminescence method for the determination of melatonin

โœ Scribed by Jianzhong Lu; Choiwan Lau; Myung Koo Lee; Masaaki Kai

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
88 KB
Volume
455
Category
Article
ISSN
0003-2670

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โœฆ Synopsis

A simple and convenient chemiluminescence (CL) method for the determination of melatonin is reported. It is based on the observation that melatonin can greatly enhance the ultra-weak CL between H 2 O 2 and acetonitrile in alkaline solution. Light emission is intense and long-lived, and even with a simple setup, a high sensitivity could be achieved. The log-log linear range was 250 nM-250 M with a 3ฯƒ detection limit of 100 nM. Singlet oxygen is suggested to be produced by the reaction between H 2 O 2 and acetonitrile and is responsible for the CL of melatonin. As a preliminary application, this simple method has been successfully applied into the determination of melatonin in health-foods.

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