## Abstract Mouse embryo cells derived in a serumβfree medium formulation (SFME cells) do not exhibit growth crisis or chromosomal abnormalities and are nontumorigenic in vivo; these cells are also reversibly growth inhibited by serum or plateletβfree plasma (Loo et al.; __Science__, 236:200β202, 1
Serum inhibition of proliferation of serum-free mouse embryo cells
β Scribed by Cathleen Rawson; Deryk Loo; Angela Helmrich; Ted Ernst; Tetsuya Natsuno; Gary Merrill; David Barnes
- Book ID
- 115892585
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 654 KB
- Volume
- 192
- Category
- Article
- ISSN
- 0014-4827
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Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are nontuniorigenic in vivo, are dependent on epidermal
We have derived serum-free mouse embryo (SFME) cultures in a basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor (EGF), high-density lipoprotein (HDL), and fibronectin. These cells are nontumorigenic, lack gross chromosomal aberrations, and exhibit several other uni