## Abstract Mouse embryo cells derived in a serumβfree medium formulation (SFME cells) do not exhibit growth crisis or chromosomal abnormalities and are nontumorigenic in vivo; these cells are also reversibly growth inhibited by serum or plateletβfree plasma (Loo et al.; __Science__, 236:200β202, 1
Karyotypic stability of Serum-Free Mouse Embryo (SFME) cells
β Scribed by Ted Ernst; Constance Jackson; David Barnes
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 844 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0920-9069
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We have derived serum-free mouse embryo (SFME) cultures in a basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor (EGF), high-density lipoprotein (HDL), and fibronectin. These cells are nontumorigenic, lack gross chromosomal aberrations, and exhibit several other uni
Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are nontuniorigenic in vivo, are dependent on epidermal
## Abstract Serumβfree mouse embryo (SFME) cells derived in a defined serumβfree medium have been cultured for more than 200 generations and display properties of neural progenitor cells. SFME cells express the neuroepithelial stem cell marker nestin in defined serumfree medium. Exposure of SFME ce