Characterization of human plasma growth inhibitory activity on serum-free mouse embryo cells
β Scribed by Patrick D. Varga Weisz; David W. Barnes
- Book ID
- 112806584
- Publisher
- Society for In Vitro Biology
- Year
- 1993
- Tongue
- English
- Weight
- 467 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1475-2689
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Serum-free mouse embryo cells, cultured in basal nutrient medium supplemented with insulin, transferrin, epidermal growth factor, fibronectin, and high-density lipoprotein, do not exhibit growth crisis, lack detectable chromosomal aberrations, are nontuniorigenic in vivo, are dependent on epidermal
## Abstract Serumβfree mouse embryo (SFME) cells derived in a defined serumβfree medium have been cultured for more than 200 generations and display properties of neural progenitor cells. SFME cells express the neuroepithelial stem cell marker nestin in defined serumfree medium. Exposure of SFME ce