Serum-free in vitro bioassay for the detection of tumor necrosis factor
β Scribed by Susan Mukavitz Kramer; Monique E. Carver
- Book ID
- 118900225
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 397 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0022-1759
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π SIMILAR VOLUMES
Synovial fluids from 6 of 12 patients with rheumatoid arthritis (RA) and from 3 of 11 patients with reactive arthritis contained measurable levels of tumor necrosis factor a (TNFa). Seven of 12 sera from RA patients contained TNFa, while only 1 of those from reactive arthritis patients was positive.
A sensitive and precise ELISA has been developed for the quantitation of recombinant Tumor Necrosis Factor-Alpha (rTNF-a) in undiluted sera. Affinity purified rabbit antibody was used as capture antibody and mouse monoclonal antibody labelled with horseradish peroxidase was used as the second antibo