𝔖 Bobbio Scriptorium
✦   LIBER   ✦

ELISA for quantitation of tumor necrosis factor-α in serum

✍ Scribed by W.S. Prince; K.J. Harder; S. Saks; B.R. Reed; A.B. Chen; A.J.S. Jones

Book ID
103910925
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
467 KB
Volume
5
Category
Article
ISSN
0731-7085

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive and precise ELISA has been developed for the quantitation of recombinant Tumor Necrosis Factor-Alpha (rTNF-a) in undiluted sera. Affinity purified rabbit antibody was used as capture antibody and mouse monoclonal antibody labelled with horseradish peroxidase was used as the second antibody in a sandwich ELISA. The assay range was from 50 to 2000 pg/ml and the relative standard deviation was 8% or less for both interassay and intra-assay precision studies. Recovery of rTNFet added to 10 different human and 10 different monkey sera ranged from 81 to 102% and 100 to 120% of the expected value, respectively.

This ELISA has been used to measure serum rTNF-oL levels in over 60 patients in Phase I Clinical Trials treated with rTNF-a. The levels in a representative, pharmacokinetic study showed low variability between 8 patients receiving intravenous bolus administration of 100 txg rTNF-edm 2. The ELISA results correlated well with TNF bioassay data with a mean specific activity of 2.5 x 107 U/rag.

📜 SIMILAR VOLUMES

Detection of tumor necrosis factor α but
Detection of tumor necrosis factor α but not tumor necrosis factor β in rheumatoid arthritis synovial fluid and serum
✍ T. Saxne; M. A. Palladino Jr; D. Heinegãrd; N. Talal; F. A. Wollheim 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 386 KB 👁 1 views

Synovial fluids from 6 of 12 patients with rheumatoid arthritis (RA) and from 3 of 11 patients with reactive arthritis contained measurable levels of tumor necrosis factor a (TNFa). Seven of 12 sera from RA patients contained TNFa, while only 1 of those from reactive arthritis patients was positive.