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Serological study of yeast killer toxins by monoclonal antibodies

โœ Scribed by Luciano Polonelli; Stefania Manzara; Stefania Conti; Giuseppe Dettori; Giulia Morace; Carlo Chezzi


Book ID
104772478
Publisher
Springer Netherlands
Year
1989
Tongue
English
Weight
877 KB
Volume
108
Category
Article
ISSN
0301-486X

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โœฆ Synopsis


Yeast killer toxins coded by determined and undetermined killer plasmids or presumptive nuclear gene(s) in various genera (Saccharomyees, Kluyveromyces, Pichia and Candida) have been serologically investigated by a monoclonal antibody (KT4), produced against the yeast killer toxin of Piehia (Hansenula) anomala UCSC 25F. Double immunodiffusion with the killer toxins as antigens and indirect immunofluorescence on whole cells of the corresponding killer yeast have been used. In both the serological procedures, monoclonal antibody KT4 proved to be reacting only with the killer toxins and the whole cells of yeasts belonging to the genus Pichia.


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