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In vitro neutralization of T-2 toxin toxicity by a monoclonal antibody

✍ Scribed by Dr. Fiorenza Minervini; Agnes Gyongyosi-Horvath; Giacomo Lucivero; Angelo Visconti; Lldiko Bama-Vetro; Lazlo Solti

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 345 KB
Volume: 2
Category: Article
ISSN: 1056-9014
DOI: 10.1002/nt.2620020304

No coin nor oath required. For personal study only.

✦ Synopsis

A T-2 toxin specific monoclonal antibody, IgGl K, with a low level of ELISA cross-reactivity to Acetyl T-2, HT-2, and is0 T-2 toxins has been produced. The ability of this monoclonal antibocly to neutralize the cytotoxicity of T-2 toxin in PHA stimulated cultures of human lymphocytes was determined by the M l l method. The complete neutralization of the toxic effect of 0.02 pM T-2 toxin was obtained with 0.03 pM of MoAb, whereas the 50% neutralizing dose ( ND, , ) was observed at 0.009 of MoAb. Partial neutralization was observed with Acetyl T-2 toxin ( ND, , = 0.038 @d) and HT-2 ( ND, = 0.94 pM). These results could represent a rational for clinical use of T-2 toxin specific monoclonal antibody in prophylaxis and therapy of T-2 toxemia.

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