A method for sequencing ODNs with both termini blocked using mass spectrometry (MS) is reported. The ladder sequencing method is based on our investigation and understanding of critical factors affecting snake venom phosphodiesterase (SVP) digestion of such ODNs. To produce sequence ladders suitable
Sequencing Regular and Labeled Oligonucleotides Using Enzymatic Digestion and Ionspray Mass Spectrometry
β Scribed by Huaiqin Wu; Clifford Chan; Hoda Aboleneen
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 189 KB
- Volume
- 263
- Category
- Article
- ISSN
- 0003-2697
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