Sequencing Oligonucleotides with Blocked
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Huaiqin Wu; Hoda Aboleneen
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Article
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2000
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Elsevier Science
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English
โ 173 KB
A method for sequencing ODNs with both termini blocked using mass spectrometry (MS) is reported. The ladder sequencing method is based on our investigation and understanding of critical factors affecting snake venom phosphodiesterase (SVP) digestion of such ODNs. To produce sequence ladders suitable