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Improved Oligonucleotide Sequencing by Alkaline Phosphatase and Exonuclease Digestions with Mass Spectrometry

โœ Scribed by Huaiqin Wu; Hoda Aboleneen


Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
93 KB
Volume
290
Category
Article
ISSN
0003-2697

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Sequencing Oligonucleotides with Blocked
โœ Huaiqin Wu; Hoda Aboleneen ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 173 KB

A method for sequencing ODNs with both termini blocked using mass spectrometry (MS) is reported. The ladder sequencing method is based on our investigation and understanding of critical factors affecting snake venom phosphodiesterase (SVP) digestion of such ODNs. To produce sequence ladders suitable