๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Sequencing Oligonucleotides by Exonuclease Digestion and Delayed Extraction Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

โœ Scribed by Igor P. Smirnov; Mark T. Roskey; Peter Juhasz; Edward J. Takach; Stephen A. Martin; Lawrence A. Haff


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
168 KB
Volume
238
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Oligonucleotide Sequencing by Fragmentat
โœ Y.F. Zhu; N.I. Taranenko; S.L. Allman; N.V. Taranenko; S.A. Martin; L.A. Haff; C ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 214 KB ๐Ÿ‘ 3 views

Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing

Ionization mechanism of oligonucleotides
โœ Yufei Kong; Yifei Zhu; Jing-Yuan Zhang ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 171 KB ๐Ÿ‘ 2 views

The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba

Analysis of solid-supported oligonucleot
โœ Albert Meyer; Nicolas Spinelli; Jean-Louis Imbach; Jean-Jacques Vasseur ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 134 KB ๐Ÿ‘ 2 views

This study presents matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) as a powerful tool to analyze and characterize oligonucleotides covalently linked to a solid support during their synthesis. The analysis of the fragment ions generated either in negative o

Phosphopeptide detection and sequencing
โœ Keiryn L. Bennett; Allan Stensballe; Alexandre V. Podtelejnikov; Marc Moniatte; ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 405 KB

## Abstract A prototype matrixโ€assisted laser desorption/ionization quadrupole timeโ€ofโ€flight (MALDIโ€QqTOF) tandem mass spectrometer was used to sequence a series of phosphotyrosineโ€, phosphothreonineโ€ and phosphoserineโ€containing peptides. The high mass resolution and mass accuracy of the instrume

Accurate peptide sequencing by post-sour
โœ Wenjun Mo; Toshifumi Takao; Yasutsugu Shimonishi ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 153 KB ๐Ÿ‘ 2 views

Partial 18 O-labeling of peptides has been applied to post-source decay experiments in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The ions which originate from the carboxyl terminus of a peptide partially retain 18 O atoms which have readily been incorporated int