Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing
Sequencing Oligonucleotides by Exonuclease Digestion and Delayed Extraction Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
โ Scribed by Igor P. Smirnov; Mark T. Roskey; Peter Juhasz; Edward J. Takach; Stephen A. Martin; Lawrence A. Haff
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 168 KB
- Volume
- 238
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba
This study presents matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) as a powerful tool to analyze and characterize oligonucleotides covalently linked to a solid support during their synthesis. The analysis of the fragment ions generated either in negative o
## Abstract A prototype matrixโassisted laser desorption/ionization quadrupole timeโofโflight (MALDIโQqTOF) tandem mass spectrometer was used to sequence a series of phosphotyrosineโ, phosphothreonineโ and phosphoserineโcontaining peptides. The high mass resolution and mass accuracy of the instrume
Partial 18 O-labeling of peptides has been applied to post-source decay experiments in a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The ions which originate from the carboxyl terminus of a peptide partially retain 18 O atoms which have readily been incorporated int