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Oligonucleotide Sequencing by Fragmentation in Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

✍ Scribed by Y.F. Zhu; N.I. Taranenko; S.L. Allman; N.V. Taranenko; S.A. Martin; L.A. Haff; C.H. Chen

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 214 KB
Volume: 11
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199705)11:8<897::aid-rcm929>3.0.co;2-z

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✦ Synopsis

Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing speed potentially can be increased. Sequence information for single strand oligonucleotide segments up to 35 mer was obtained from fragmentation patterns using a mixture of anthranilic acid and ammonium citrate or 2,4,6-trihydroxyacetophenone and ammonium citrate as matrices. This method shows that the cleavage of P -O bond at 5'-linkage and C -O bond at 3'-linkage could be established in the MALDI process. Such cleavages at specific sites in the backbone of oligonucleotide can provide the foundation for fast oligonucleotide sequencing.

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