Sequence-specific inhibition of RNA elongation by actinomycin D
β Scribed by V.A. Aivasashvilli; R.Sh. Beabealashvilli
- Book ID
- 115915068
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 566 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0014-5793
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π SIMILAR VOLUMES
## Abstract A quantitative doseβresponse study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine.
Preferential inhibition of the synthesis of ribosomal RNA by low doses of actinomycin D was used to investigate the quantitative relationship between the intensity of silver staining of nucleoli and the rate of RNA-synthesis. The two parameters were found to be strongly correlated in human diploid f