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Differential inhibition of RNA synthesis by actinomycin

✍ Scribed by Klenow, Hans; Frederiksen, Sune

Book ID
122641531
Publisher
Elsevier Science
Year
1964
Tongue
English
Weight
248 KB
Volume
17
Category
Article
ISSN
0006-291X

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Inhibition of RNA synthesis by actinomyc
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## Abstract A quantitative dose‐response study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine.

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Preferential inhibition of the synthesis of ribosomal RNA by low doses of actinomycin D was used to investigate the quantitative relationship between the intensity of silver staining of nucleoli and the rate of RNA-synthesis. The two parameters were found to be strongly correlated in human diploid f