HRS60.1, a monomer unit (184 bp) of a highly repeated nuclear DNA sequence of Nicotiana tabacum, has been cloned and sequenced. Following BamHI digestion of tobacco DNA, Southern hybridization with HRS60.1 revealed a ladder of hybridization bands corresponding to multiples of the basic monomer unit.
Sequence characteristics of a cervid DNA repeat family
β Scribed by Sohail A. Qureshi; R. D. Blake
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 389 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0022-2844
No coin nor oath required. For personal study only.
β¦ Synopsis
The (G + C) distribution and the presence and amounts of repetitive sequence families in the whitetailed deer (Odocoileus virginianus) have been examined. The distribution ranges from 20 to 70% (G + C) and shows four distinct repeat families. A 0.7-kb family, DII, corresponds to satellite II in domestic bovids--ox, sheep, and goat--and was singled out for detailed characterization. DII has a prototypic repeat of 67% (G + C), consists of 25,000 tandem copies, and contributes 1.7% to the genomic DNA. Sequencing and electrophoretic analysis indicate a repeat length of 691 bp. These characteristics are similar to those of the bovid satellite II families as well as to those of other cervids that we have examined.
π SIMILAR VOLUMES
Generation of Simple Sequence Repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequen