Methodology of generation and characteristics of simple sequence repeat DNA markers in avocado (Persea americanaM.)

✍ Scribed by Uri Lavi; Mahinur Akkaya; Arvind Bhagwat; Emanuel Lahav; Perry B. Cregan

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 632 KB
• Volume: 80
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00039648

✦ Synopsis

Generation of Simple Sequence Repeat (SSR) DNA markers was based on the construction of genomic DNA library of avocado (Persea americana M.). The library was screened with the four dinucleotide probes (AG), (AT), (GC) and (CA). Positive clones were sequenced to validate the presence of simple sequence repeats (SSR) and to generate polymerase chain reaction (PCR) primers based on the sequences flanking the simple sequence repeat. Twenty six different pairs of primers which yield a PCR product in the initial screening were synthesized. The SSR AlE11 was found to have eleven alleles while A3F8 has eight alleles. The SSRs in avocado were found to be inherited in a Mendelian fashion.