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Sequence and diversity of variable gene segments coding for rabbit T-cell receptor beta chains

✍ Scribed by Takahiro Isono; Yuji Isegawa; Akira Seto

Publisher: Springer-Verlag
Year: 1994
Tongue: English
Weight: 535 KB
Volume: 39
Category: Article
ISSN: 0093-7711
DOI: 10.1007/bf00188786

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✦ Synopsis

The nucleotide sequences of 11 variable gene segments coding for rabbit T-cell receptor beta (Tcrb-V) chains were determined by directly sequencing fragments amplified by the cassette-ligation mediated polymerase chain reaction (CLM-PCR) and by modified anchor PCR without the cloning procedure. The nucleotide sequences in two of these 11 rabbit Tcrb-V gene segments coincided with those in two of the four rabbit Tcrb-V gene segments previously reported; the others have not been described. The percentage similarity of each nucleotide sequence of the 11 rabbit Tcrb-V gene segments was analyzed and the segments were divided into nine families, which were homologous to nine human families (Vb 2, 3, 4, 5, 7, 8, 10, 18, and 22), respectively.

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