The nucleotide sequences of 11 variable gene segments coding for rabbit T-cell receptor beta (Tcrb-V) chains were determined by directly sequencing fragments amplified by the cassette-ligation mediated polymerase chain reaction (CLM-PCR) and by modified anchor PCR without the cloning procedure. The
Sequence and expression of a novel human T-cell receptor β-chain variable gene segment subfamily
✍ Scribed by Richard K. Wilson; Eric Lai; Leonard D. H. Kim; Leroy Hood
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 663 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0093-7711
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We have studied the diversity of the expressed human T-cell receptor (TCR) beta-chain repertoire by analysis of mRNA from unstimulated peripheral blood T-cells. The anchored-polymerase chain reaction (PCR) was used to isolate TCRB transcripts. Of 20 full or near full-length functional transcripts se
The nucleotide sequences of 38 T-cell receptor (Tcr) beta-chain cDNA clones which were isolated from a cDNA library (2 x 10(6) plaques) constructed from bovine peripheral blood lymphocytes were determined. Of 38 cDNA clones, 22 were rearranged and contained the functional variable (V) gene segments.