An isocratic reversed-phase high-performance liquid chromatographic system is described for the separation of a relatively large number (> 20) of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides. A 25 cm x 5 mm ODS-Hypersil (5 pm particle size) column was used with m
Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography
β Scribed by Colin L. Willis; Chang Kee Lim; Timothy J. Peters
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 406 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0731-7085
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Chromatography of AMP, NAD+, or NADH on a reverse-phase C,, Porasil B column rapidly removes ammonium formate or potassium phosphate from 90% of the nucleotide. Earlier reports showed these salts could not be separated from nucleotides by conventional desalting using gel filtration.
Reversed-phase high-performance liquid chromatography using a C,8 column with volatile buffers as the eluant was applied to the separation of a number of nucleosides and nucleotides. Groups of seven nucleosides and five nucleoside monophosphates were separated isocratically employing 0.1 M trimethyl