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Isocractic reversed-phase high-performance liquid chromatography of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides

โœ Scribed by C.K. Lim; T.J. Peters

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
455 KB
Volume
461
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

An isocratic reversed-phase high-performance liquid chromatographic system is described for the separation of a relatively large number (> 20) of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides. A 25 cm x 5 mm ODS-Hypersil (5 pm particle size) column was used with methanol-triethylammonium phosphate buffer as eluent. The effects of methanol content, pH, and ionic strength of the buffer on retention and resolution of the nucleotides have been studied. The applicability of the system was demonstrated by the analysis of nucleotides in cells and tissue extracts.

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