Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells
β Scribed by Jones, Richard J.; Wagner, John E.; Celano, Paul; Zicha, Milada S.; Sharkis, Saul J.
- Book ID
- 109765820
- Publisher
- Nature Publishing Group
- Year
- 1990
- Tongue
- English
- Weight
- 258 KB
- Volume
- 347
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/347188a0
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## Abstract Mouse bone marrow cells have been cultured in diffusion chambers and their capacity to form spleen colonies in irradiated mice investigated after different culture periods. The number of spleen colonyβforming units (CFU) in the chambers decreased during the first day of culture. The num
Mouse bone marrow cells in suspension were separated into a number of fractions on the basis of cell density by equilibrium density gradient centrifugation, or on the basis of cell size by velocity sedimentation. After each type of separation, the cells from the various fractions were assayed for th
Hemopoietic colony formation in agar occurred spontaneously in mass cultures of marrow cells obtained from a number of species (guinea pig, rat, lamb, rabbit, pig, calf, human and Rhesus monkey). This contrastcd with the observation that colony formation by mouse bone marrow exhibited an absolute re