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Physical separation of colony stimulating cells from in vitro colony forming cells in hemopoietic tissue

✍ Scribed by M. A. S. Moore; N. Williams

Publisher: John Wiley and Sons
Year: 1972
Tongue: English
Weight: 940 KB
Volume: 80
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040800206

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✦ Synopsis

Hemopoietic colony formation in agar occurred spontaneously in mass cultures of marrow cells obtained from a number of species (guinea pig, rat, lamb, rabbit, pig, calf, human and Rhesus monkey). This contrastcd with the observation that colony formation by mouse bone marrow exhibited an absolute requirement for an exogenous source of a colony stimulating factor. Analysis of spontaneous colony formation in Rhesus monkey marrow cultures revealed the presence of a cell type in hemopoietic tissue, capable of elaborating colony stimulating factor when used to condition media or as feeder layers. Equilibrium density gradient centrifugation separated colony stimulating cells from in vitro colony forming cells in monkey bone marrow. Separation studies on spleen, blood and marrow characterized the stimulating cells as of intermediate density, depleted or absent in fractions enriched for cells of the granulocytic series and localized in regions containing lymphocytes and monocytes. Adherence column separation of peripheral blood leukocytes showed the stimulating cells to be actively adherent, unlike the majority of lymphocytes, and combined adherence column and density separation indicated that stimulating cells were present in hemopoietic tissue within the population of adherent lymphocytes or monocytes. '71; Moore et al., '72) or to stimulate colony formation when prepared as "feeder layers" (Bradley and Metcalf, '66; Pike and Robinson, '70).

Studies on in vitro culture of Rhesus

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