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Diffusion chamber and spleen colony assay of murine haematopoietic stem cells

✍ Scribed by Harald Breivik; Haakon Breien Benestad; Arne Bøyum

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
612 KB
Volume
78
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Mouse bone marrow cells have been cultured in diffusion chambers and their capacity to form spleen colonies in irradiated mice investigated after different culture periods. The number of spleen colony‐forming units (CFU) in the chambers decreased during the first day of culture. The number then increased rapidly to a level significantly above the original chamber value on the third to fifth day of culture. By that time large numbers of granulocytes and macrophages had also appeared.

Histological examination of spleen colonies showed that prior culturing did not alter the ratio between the different types of colonies.

Cultured bone marrow cells which were transferred to new chambers retained granulopoietic capacity. This capacity increased between the first and second day of primary culturing. At this time hydroxyurea injections to chamber hosts revealed that the progenitor cells were proliferating.

The results show that the granulopoietic progenitor cells of the chambers are stem cells, and that one progenitor cell type is identical with the CFU.

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