Separation of myocardial purine nucleosides and nucleotides by one-dimensional thin-layer chromatography

✍ Scribed by Diane K. Reibel; Michael J. Rovetto

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 324 KB
• Volume: 161
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85263-8

✦ Synopsis

A number of reports have appeared concerning separation of purine and pyrimidine nucleosides and nucleotides by thin-layer chromatography (TLC) on polyethyleneimine (PEI) cellulose l--6. These represent modifications of the basic techniqu'es developed by Randerath and Randerath'-g who first employed this resin for such separations_ Nucleosides and nucleotides in biological fluids have been separated on PElzsJ, but with few exceptions 3v5 these techniques were not applied to separation and quantification of nucleotides and nucleosides in tissue extracts. Moreover, methods for simultaneous separation of adenine nucleotides and nucleosides have previously relied on two-dimensional chromatography. Development of the TLC plates in a second dimension Featly restricts the number of samples per plate and significantly prolongs the time required for analysis.

We have employed one-dimensional development with a solvent system which enables simuhaneous separation of adenine nucleotides and nucleosides and their quantification in myocardial tissue extracts.