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Separation of mannosylretinylphosphate from dolichylmannosylphosphate by chromatography on columns of DEAE-Sephacel

✍ Scribed by Wlodzimierz Sasak; Carol S. Silverman-Jones; Luigi M. De Luca

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 315 KB
Volume: 97
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90075-7

No coin nor oath required. For personal study only.

✦ Synopsis

A one-step column chromatographic procedure on DEAE-Sephacel allows the separation of mannosylretinylphosphate from dolichylmannosylphosphate with minimal breakdown of the mannosyhetinylphosphate. Using this procedure, subcellular fractions of rat liver were shown to be active in synthesizing both mannolipids from GDP-[Wlmannose in the absence or presence of exogenous retinylphosphate.

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