Aeromonas sp. ACY 95 produces constitutively and intracellularly a penicillin V acylase at an early stage of fermentation (12 h) and a cephalosporin C acylase at a later stage (36 h). Some penicillins, cephalosporin C and their side chain moieties/analogues, phenoxyacetic acid, penicillin V and peni
Separation of cephalosporin C acylase and penicillin V acylase from Aeromonas ACY 95 by chemical treatment
β Scribed by S.S. Ambedkar; B.S. Deshpande; J.G. Shewale
- Book ID
- 114080702
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 242 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1873-3298
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Different techniques including toluene-ethanol, guanidine hydrochloride, guanidine hydrochloride-EDTA, lysozyme, lysozyme-EDTA and sonication treatments were used to extract penicillin G acylase from Escherichia coli ATCC 11105 cells. The obtained results show that penicillin G acylase extraction by
A new chemoenzymatic synthesis of Cefazolin through the correct assembly of three bioU'ansformations catalysed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in fully aqueous medium wit