✦ LIBER ✦
Chemoenzymatic one-pot synthesis of cefazolin from cephalosporin C in fully aqueous medium, involving three consecutive biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase
✍ Scribed by Roberto Fernández-Lafuente; JoséM. Guisán; Massimo Pregnolato; Marco Terreni
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- French
- Weight
- 190 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0040-4039
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✦ Synopsis
A new chemoenzymatic synthesis of Cefazolin through the correct assembly of three bioU'ansformations catalysed by D-aminoacid oxidase, glutaryl acylase and penicillin G acylase is described. This multienzymatic synthesis has been performed from the natural Cephalosporin C in fully aqueous medium without intermediate purification stages. Almost quantitative yields have been achieved in all the enzymatic reactions.