Separation of basic central nervous system drugs by capillary electrochromatography

✍ Scribed by Jan Jiskra; Henk A. Claessens; Carel A. Cramers

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 301 KB
• Volume: 26
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200390013

✦ Synopsis

Abstract

This article describes the sample preparation and separation of three strongly basic central nervous system (CNS) drugs by capillary electrochromatography. The separation was developed on Hypersil C8 MOS and Hypersil Phenyl stationary phases using acetonitrile (ACN) as organic modifier together with mobile phase additives, ammonia, ethylenediamine, or 1,3‐diaminopropane. A successful, fast separation after a simple derivatization procedure of the compound was achieved on the Hypersil Phenyl stationary phase using ethylenediamine as mobile phase additive. The general approach to method development for complex mixtures is discussed.