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Separation of angiotensins by high-performance liquid chromatography on a weak anion-exchange bonded phase

✍ Scribed by Miral Dizdaroglu; Henry C. Krutzsch; Michael G. Simic

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
309 KB
Volume
123
Category
Article
ISSN
0003-2697

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✦ Synopsis

A mixture of 12 angiotensins was separated by high-performance liquid chromatography on a weak anion-exchange bonded phase using a triethylammonium acetate buffer and acetonitrile as the eluant. An excellent separation of these compounds was obtained. Recoveries for all 12 were over 90%, as determined by comparative amino acid analysis. A strong effect of temperature on retention was observed.

The buffer used here allows sensitive detection of these peptides at wavelengths in the range 210-225 nm. Because the eluants are also volatile, isolation of separated compounds for reuse or further analysis is facilitated.

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