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Separation of lipid classes from plant tissues by high performance liquid chromatography on chemically bonded stationary phases

✍ Scribed by Christie, William W. ;Anne Urwin, R.

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
358 KB
Volume
18
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

The wide range of lipid classes found in plant tissues, including simple lipids, glycolipids, and phospholipids, have been resolved by gradient high performance liquid chromatography with evaporative light‐scattering detection. The lipids from potato tubers were used to optimize the separations. Adsorption chromatography with silica gel gave disappointing results for the plant glycolipids. Much better separations were obtained with chemically bonded stationary phases, e.g. those with propionitrile or polymeric vinyl alcohol as the functional moiety. With careful calibration, good reproducibility was possible. Only highly acidic lipids such as phosphatidylserine still present some problems.

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