Separation and quantification of histamine and Nτ-methylhistamine in brain extracts
✍ Scribed by Manel Pastó; Josefa Sabria
- Book ID
- 101757990
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 300 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0269-3879
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
We have developed a liquid chromatographic method which uses electrochemical detection for the simultaneous quantitation of histamine and N'-methylhistamine in rat brain. The amines are derivatized with the water-soluble Bolton-Hunter reagent (sulfo B-H). Perchloric acid extracts of rat brains are c
A simple nonradioisotopic fluorescent method for assay of histamine N-methyltransferase (HMT) activity was developed. After termination of the HMT reaction, the remaining excess substrate, histamine, was degraded by Pauly 's diazo reagent, whereas the product, N tau-methylhistamine (N- MeHA ), was n