The differential reaction of histamine and Nτ-methylhistamine with Pauly's diazo reagent: Application to assay of histamine N-methyltransferase activity
✍ Scribed by Ikuo Imamura; Kazutaka Maeyama; Takehiko Watanabe; Hiroshi Wada
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 343 KB
- Volume
- 139
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simple nonradioisotopic fluorescent method for assay of histamine N-methyltransferase (HMT) activity was developed. After termination of the HMT reaction, the remaining excess substrate, histamine, was degraded by Pauly 's diazo reagent, whereas the product, N tau-methylhistamine (N- MeHA ), was not degraded by the reagent. Then the mixture was applied to high-performance liquid chromatography under conditions in which N- MeHA was not separated from histamine, and N- MeHA was measured fluorometrically by condensation with o-phthalaldehyde. The method would be convenient for measurement of HMT activity during enzyme purification.