Analysis of the dose-response relationship of histamine and Nτ-methylhistamine
✍ Scribed by L. Stanovnik; F. Erjavec
- Book ID
- 105416717
- Publisher
- SP Birkhäuser Verlag Basel
- Year
- 1982
- Tongue
- English
- Weight
- 287 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1420-908X
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A simple nonradioisotopic fluorescent method for assay of histamine N-methyltransferase (HMT) activity was developed. After termination of the HMT reaction, the remaining excess substrate, histamine, was degraded by Pauly 's diazo reagent, whereas the product, N tau-methylhistamine (N- MeHA ), was n
We have developed a liquid chromatographic method which uses electrochemical detection for the simultaneous quantitation of histamine and N'-methylhistamine in rat brain. The amines are derivatized with the water-soluble Bolton-Hunter reagent (sulfo B-H). Perchloric acid extracts of rat brains are c
A method for the simultaneous determinations of histamine and its metabolite N tau-methylhistamine by HPLC-chemiluminescence coupled with immobilized diamine oxidase was developed. The method was based on the determination of chemiluminescence formed by the reaction of a luminol-ferricyanide mixture