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Separation and purification of harmine and harmaline from Peganum harmala using pH-zone-refining counter-current chromatography

✍ Scribed by Xiao Wang; Yanling Geng; Daijie Wang; Xingang Shi; Jianhua Liu

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
544 KB
Volume
31
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

pH‐zone‐refining counter‐current chromatography was successfully applied to the separation of alkaloids from a crude extract of Peganum harmala L. using a multilayer coil planet centrifuge. The experiment was performed with a two‐phase solvent system composed of methyl tert‐butyl ether/THF/water (2:2:3 by volume) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5 mM) to the aqueous mobile phase as an eluter. From 1.2 g of the crude extract, 554 mg harmine and 325 mg harmaline were obtained each with a purity of over 96% as determined by HPLC. The structures of the isolated compounds were identified by electron ionization MS (EI‐MS), ^1^H NMR, and ^13^C NMR.

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