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Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high-speed counter-current chromatography

✍ Scribed by Shao Liu; Bin Wang; Xin-Zhong Li; Long-feng Qi; Yi-Zeng Liang

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
547 KB
Volume
32
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A preparative high‐speed counter‐current chromatography method for separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN was successfully established by using n‐hexane‐ethyl acetate‐methanol‐water (5:8:4:5, v/v, containing 0.5% NH~4~OH) as the two‐phase solvent system. From 200 mg of crude extract, 18.4 mg of liensinine, 19.6 mg of isoliensinine and 58.4 mg of neferine were obtained with the purity of 96.8, 95.9, and 98.6%, respectively. The identification of the three alkaloids was performed with ^1^H NMR and ^13^C NMR.

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