Preparative separation of atropine and scopolamine from Daturae metelis Flos using pH-zone-refining counter-current chromatography with counter-rotation and dual-mode elution procedure
✍ Scribed by Yutong He; Jianguang Luo; Lingyi Kong
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 200 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
To isolate atropine and scopolamine from Daturae metelis Flos, three different elution modes have been applied in pH‐zone‐refining counter‐current chromatography. These separations were performed with a two‐phase solvent system composed of ethyl acetate/n‐butanol/water (4:1:5 v/v) with 0.50% triethylamine in the organic phase and 0.15% hydrochloric acid in the aqueous phase. As a result, the best separation was obtained by counter‐rotation and dual‐mode elution procedure. In this new separation mode, the mobile phase and stationary phase were exchanged when the rotation direction was reversed. The two purified alkaloids (purity over 98% as determined by HPLC) were identified by ESI‐MS, ^1^H‐NMR and ^13^C‐NMR.