𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Separation and analysis of 4′-epimeric UDP-sugars by borate high-performance liquid chromatography

✍ Scribed by Gisbert Weckbecker; Dietrich O.R. Keppler

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
687 KB
Volume
132
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Separation of UDP-glucose from UDP-galactose, of UDP-N-acetylglucosamine from UDP-N-acetylgalactosamine, of UDP-glucuronate from UDP-galacturonate, or of UDP-glucosamine from UDP-galactosamine was achieved within lo-45 min by isocratic anion-exchange highperformance liquid chromatography (HPLC) using a flow rate of 2 ml/min. The eluants were composed of borate as complex-forming and eluting agent and of glycerol for protection of the alkali-labile silica packing of the column. This borate HPLC was suitable for the analysis of 4'epimeric UDP-sugars in the range of 2 to 100 nmol. The applicability of this technique was demonstrated by determination of the relative amounts of 4'epimeric UDP-amino sugars formed in rat liver after administration of Dgalactosamine. Since a high salt content of UDP-sugar samples can interfere with borate HPLC, desalting was performed on a l-ml C,s cartridge using triethylammonium hydrogen carbonate buffer. This procedure enabled the complete separation of various nucleotides from salts within 10 min prior to HPLC.

📜 SIMILAR VOLUMES

Separation and Analysis of 4′-Epimeric U
Separation and Analysis of 4′-Epimeric UDP-Sugars, Nucleotides, and Sugar Phosphates by Anion-Exchange High-Performance Liquid Chromatography with Conductimetric Detection
✍ S.R. Hull; R. Montgomery 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 424 KB

An anion-exchange HPLC method coupled with conductimetric detection was developed for the analysis of UDP-sugars, nucleotides, and sugar phosphates, each in a single chromatographic run. The analysis was applicable to concentrations over 50 pmol. The utility of this technique was demonstrated by the

Separation and Analysis of 4′-Epimeric U
Separation and Analysis of 4′-Epimeric UDP-Sugars by Ion-Pair Reversed-Phase HPLC
✍ R. Lagunas; J.C. Diezmasa 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 536 KB

A simple and sensitive method for determination of 4'-epimeric UDP-sugars using ion-pair reversed-phase HPLC has been developed. The method presents advantages over existing ion-exchange HPLC procedures mainly concerning sensitivity and rapidity of analysis as well as efficiency and stability of the

Separation of Glycopeptides by High Perf
Separation of Glycopeptides by High Performance Liquid Chromatography
✍ Marsha Rich Rosner; Phillips W. Robbins 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 636 KB

## Abstract A method is presented for separation of tryptic glycopeptides‐containing oligosaccharides of the N‐asparagine‐linked type. High performance liquid Chromatography (HPLC) of glycopeptides on a C18 reverse‐phase system eluted with a gradient of 0%–50% acetonitrile in 0.1 M NaPO~4~ pH 2.2 r

Analysis of reducing sugars as their chr
Analysis of reducing sugars as their chromophoric hydrazones by high-performance liquid chromatography
✍ Koji Muramoto; Rina Goto; Hisao Kamiya 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 602 KB

A chromophoric hydrazide, 4'-N,N-dimethylamino-4-azobenzene sulfonyl hydrazide (DABS-hydrazide), was prepared from 4'-N,N-dimethylamino-4-azobenzene sulfonyl chloride by reaction with hydrazine. Reducing sugars were derivatized with DABS-hydrazide at 50 degrees C for 120 min. The chromophoric hydraz

Microquantitative analysis of neutral an
Microquantitative analysis of neutral and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography
✍ Hiroshi Takemoto; Sumihiro Hase; Tokuji Ikenaka 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 425 KB

A method for the quantitation of picomole amounts of neutral and amino sugars in glycoconjugates was developed. Glycoconjugates were hydrolyzed with a mixture of equal amounts of 4 M trifluoroacetic acid and 4 M hydrochloric acid, and the free amino groups were acetylated. Sugars were coupled with 2