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Separation and Analysis of 4′-Epimeric UDP-Sugars, Nucleotides, and Sugar Phosphates by Anion-Exchange High-Performance Liquid Chromatography with Conductimetric Detection

✍ Scribed by S.R. Hull; R. Montgomery

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
424 KB
Volume
222
Category
Article
ISSN
0003-2697

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✦ Synopsis

An anion-exchange HPLC method coupled with conductimetric detection was developed for the analysis of UDP-sugars, nucleotides, and sugar phosphates, each in a single chromatographic run. The analysis was applicable to concentrations over 50 pmol. The utility of this technique was demonstrated by the measurement of UDP-sugar 4'-epimerase activity of cell-free extracts from wild-type and mutant Neisseria meningitidis serogroup B strains. Additionally, the method has been applied to the analysis of the intermediates of glycolysis in human erythrocytes. 1994 Academic Press, Inc.

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