Sensitivity of cultured human pancreatic carcinoma cells to dihydroxyanthracenedione

✍ Scribed by George Fountzilas; Howard Gratzner; Lori O. Lim; Adel A. Yunis

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: French
• Weight: 579 KB
• Volume: 33
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910330311

✦ Synopsis

We tested the effectiveness of dihydroxyanthracenedione (DHAD) on cell growth of two human pancreatic carcinoma cell lines MIA PaCa-2 and PANC-1. At the level of ID,,, the drug was almost equally effective against both cell lines. When the time exposure of MIA PaCa-2 cells to the drug was increased from I h to continuous exposure for 5 days, the ID,, was decreased about three-fold only (I .4 X lo4 M and 4 X lo4 M respectively). At the level of ID,, also the difference between 6 h exposure and continuous exposure for 5 days was minimal. In equimolar concentrations and with I h exposure, DHAD was more effective against MIA PaCa-2 cells than other chemotherapeutic agents including adriamycin, mitomycin-C, 5-FU, vincristine, vindesine. vinblastine, VP-16-2 13, bleomycin, cis-platinum, asparaginase and acivicin. In concentrations of 5 X lo-' M, DHAD caused about 40 % inhibition of "C-thymidine incorporation of MIA PaCa-2 cells. Treatment of MIA PaCa-2 cells with the ID, of DHAD for I h caused retardation of cellular traverse, with the major effect appearing to be in G2+M phase of the cycle. From these data DHAD appears to be a potent drug against human pancreatic carcinoma in V i m .