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Human pancreatic carcinoma (mia paca-2) in continuous culture: Sensitivity to asparaginase

✍ Scribed by Adel A. Yunis; Grace K. Arimura; David J. Russin

Publisher: John Wiley and Sons
Year: 1977
Tongue: French
Weight: 636 KB
Volume: 19
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910190118

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✦ Synopsis

Abstract

An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

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✍ Keiko Ishino; Hidesuke Fukazawa; Mayumi Shikano; Motoi Ohba; Toshio Kuroki; Yosh 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 222 KB

## Abstract We found that 12‐__O__‐tetradecanoylphorbol‐13‐acetate (TPA) promoted anchorage‐independent growth but did not affect anchorage‐dependent growth of MIA PaCa‐2 human pancreatic carcinoma cells. TPA markedly activated mitogen‐activated protein kinase (MAPK)/extracellular signal–regulated