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Sensitive Electrochemical Determination of Unlabeled MutS Protein and Detection of Point Mutations in DNA

✍ Scribed by Paleček, Emil; Masařík, Michal; Kizek, Rene; Kuhlmeier, Dirk; Hassmann, Jörg; Schülein, Jürgen


Book ID
126114716
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
138 KB
Volume
76
Category
Article
ISSN
0003-2700

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A novel strategy is described for highly sensitive DNA detection and point mutation identification based on the combination of reverse molecular beacon with DNA ligase. A 5'-phosphoryl and 3'-ferrocene terminated DNA sequence is used as detection probe, which may be ligated to capture DNA immobilize