Sensitive determination of the benzene metabolite S-phenylmercapturic acid in urine by high-performance liquid chromatography with fluorescence detection
β Scribed by T. Einig; W. Dehnen
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 284 KB
- Volume
- 697
- Category
- Article
- ISSN
- 1873-3778
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## Abstract A simple, specific and sensitive HPLC method has been developed for the determination of metoprolol in human plasma and urine. Separation of metoprolol and atenolol (internal standard) was achieved on an Ace C~18~ column (5βΞΌm, 250βmmΓ4.6βmm id) using fluorescence detection with Ξ»~ex~=2
Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co