Determination of metoprolol in human plasma and urine by high-performance liquid chromatography with fluorescence detection

✍ Scribed by Bilal Yilmaz; Ali Asci; Sakir Arslan

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 143 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000136

✦ Synopsis

Abstract

A simple, specific and sensitive HPLC method has been developed for the determination of metoprolol in human plasma and urine. Separation of metoprolol and atenolol (internal standard) was achieved on an Ace C~18~ column (5 μm, 250 mm×4.6 mm id) using fluorescence detection with λ~ex~=276 nm and λ~em~=296 nm. The mobile phase consists of methanol–water (50:50, v/v) containing 0.1% TFA. The analysis was performed in less than 10 min with a flow rate of 1 mL/min. The assay was linear over the concentration range of 3 – 200 and 5 – 300 ng/mL for plasma and urine, respectively. The LOD were 1.0 and 1.5 ng/mL for plasma and urine, respectively. The LOQ were 3.0 and 5.0 ng/mL for plasma and urine, respectively. The extraction recoveries were found to be 95.6 ± 1.53 and 96.4 ± 1.75% for plasma and urine, respectively. Also, the method was successfully applied to three patients with hypertension who had been given an oral tablet of 100 mg metoprolol.